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Michaelis Menten Equation Calculator

maximum velocity
Michaelis constant

Result

Calculated result 50 Based on the values entered.
↗ Chemistry

Michaelis Menten Equation Calculator Calculate the initial reaction velocity of an enzyme-catalyzed reaction using the Michaelis-Menten equation. — free online calculator.

Formula

v = Vmax × [S] / (Km + [S])
  • Michaelis Menten Equation Calculator calculates its result from Vmax (maximum velocity), Km (Michaelis constant), Substrate Concentration. It uses the formula v = Vmax × [S] / (Km + [S]). Change any input to recalculate the result instantly.
  • The main inputs are Vmax, Km, Substrate Concentration. Enter them in the units and format shown in the calculator.
  • Use the units, labels and assumptions shown beside each input so the formula is applied consistently.
  • Changing any input above recalculates the result with the same calculation engine used by this page.

Example: Michaelis Menten Equation

Inputs
  • Vmax: 100 maximum velocity
  • Km: 5 Michaelis constant
  • Substrate Concentration: 5

Using Vmax (maximum velocity) = 100, Km (Michaelis constant) = 5, Substrate Concentration = 5, the calculated result is 50.

Sample result: Calculating…

Frequently asked questions

What does the Michaelis Menten Equation calculate?

Michaelis Menten Equation Calculator Calculate the initial reaction velocity of an enzyme-catalyzed reaction using the Michaelis-Menten equation. — free online calculator.

Which values should I enter?

Enter Vmax, Km, Substrate Concentration using the units and format shown beside each field.

What formula does this calculator use?

The calculator uses the following relationship: v = Vmax × [S] / (Km + [S]).

Why might my result differ from another source?

Results can differ when another source uses different units, rounding rules, conventions or assumptions. Compare the inputs and definitions before comparing results.

Does the result update automatically?

Yes. Changing an input updates the result using the same calculation engine shown on this page.

Page information

Updated: October 7, 2026